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2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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Chardakov Method for Determining Water Potential. The Chardokov method provides a quick means to determine plant tissue water potentials. This method depends on the change in density in a solution that occurs after a tissue has been immersed in it. The solution gains or looses water depending on the water potential of the tissue.

If the density of a solution does not change no net movement of water then this solution has the same water potential as the tissues that were incubated in it.

Chardakov method Essay – Free Papers and Essays Examples

Charda,ov is assumed that solute movement between tissue and solution is negligible. Density chardzkov can be observed by watching whether a drop of the original solution floats or sinks in the test solution after tissue incubation. Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used. What is the water potential of potato tissue?


Dispense 10 mL of water or a sucrose solution 0. Use a cork borer to prepare at least 27 uniform tissue samples from the potato.

Chardakov method

Cut them to the same length ca. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel.

Put two or preferably three potato cores in each solution water or sucrose. If necessary, add more of the appropriate solution to completely submerge the cores but the final volume in each tube must be the same.

Incubate the cores for at least 1.

Periodically swirl the containers. Pour off the solutions into a set of empty, correspondingly labeled tubes. Mix the tubes thoroughly with a vortex mixer.

Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and then mix. Immerse the pipette chatdakov the water that previously had tissue sections in it until the tip is approximately at the center of the tube.


Investigating osmosis: measuring the water potential of a potato cell

Slowly release a drop of the methylene blue solution from the pipette and note whether the drop of the dye sinks, disperses, or floats to the surface in this solution and subjectively estimate whether it does so rapidly or slowly. Record your results Table 2 and repeat this procedure for each of the sucrose solutions. Be sure to use a different pipet for each dye stock.

Complete Tables 1 and 2. Determine the approximate sucrose concentration for which there is no net change in density after tissue incubation i. The answer will be in Jm -3 which are equivalent to pressure Pa. Divide by 10 6 to convert to MPa. Response of drops float, sink, hover when placed in solutions in which potato cores have been incubated.