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Efectividad antimicótica de soluciones quelantes usadas en endodoncia: Estudio comparativo in vitro de la eficacia antimicótica de EDTA 17% y MD Cleanser. Estudio in vitro del grado de erosión que provoca el EDTA sobre la dentina .. Canalda C, Brau E. Endodoncia, técnicas clínicas y bases cientí- ficas. Editorial. Limpiar y eliminar saliva,. sangre y posibles restos. de materiales extraños,. principalmente lubrica. CLASIFICACIÓN: Biocompatible. SUERO.

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Forty-eight single-rooted teeth were used in this study. Data were analysed by means of the Kruskal-Wallis and Mood’s median tests. Chelating agent; Citric acid; Edetic acid; Egtazic acid.

Ethylenediaminetetraacetic acid in endodontics

Mechanically prepared dentin surfaces are always covered with the so-called smear layer, a loosely bonded amorphous layer of organic and inorganic debris. Thus, several chelating agents and acids have been used to remove this layer, with varying degrees of success.

These irrigating solutions have been chosen due to their direct action over calcium ions. The calcium present in hydroxyapatite crystals is one of the main inorganic elements of dentin. Any change in the calcium ratio can significantly alter ensodoncia original proportion of organic and inorganic components, which can alter dentin permeability, microhardness and solubility.

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Although EDTA has a long-standing history as the agent of choice in Endodontics, researchers have reported its irritating potential 7, Recently, EGTA has also been recommended for root canal biomechanical preparation, since it pre-sents a more precise action on calcium ions than EDTA 1,2,9,12, Another chelating agent, CDTA, has also been reported to significantly bind the divalent cation.

Recent studies have investigated the application of both chelating agents endodoncja an alternative solution to remove the smear layer. In the study of Cruz-Filho et al. No study has yet compared all chelating agents using the same concentration.

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Endodincia acid has also been studied for its ability as a decalcifying and cleansing solution in root canal irrigation 8,13,14, However, in some scanning electron microscope studies, it was observed that citric acid left precipitated crystals in the root canal walls 3,4. It is a biological and organic acid, with sufficient tissue compatibility 6,7,10,11, Studies have demonstrated that the pH and concentration of demineralising solutions are important factors to be considered in their action in removing surface smear layer 4,5.


A literature review has revealed that different dndodoncia, concentrations, pH, working time and methods have been used to evaluate the irrigating solutions. The pH of the test solutions was 7.


Endodobcia materials used were: All test solutions were freshly prepared. The salts were weighed and diluted in deionised water Permution E.

The molar concentrations of the acid solutions were: Forty-eight single-rooted freshly extracted human teeth with straight root canals and similar length were used in this study. The anatomical crowns were removed transversally at the cemento-enamel junction, and the pulp tissue was extirpated.

The working length of all teeth was established by passing a 15 K-file endodonciaa the apical foramen and then reducing the length by 1. The canals were instrumented by the step-back technique to 70 K-file Dentsply Maillefer, Ballaigues, Switzerland with the minimum master apical file The present results are in agreement endodobcia those of Hennequin et al. According to both previous studies, the pH of citric acid solution has been shown to be a more important factor to demineralisation than concentration.

These chelating agents react with calcium ions in the hydroxyapatite crystals to produce a metallic chelate, and this process is characterised by protonation and complex formation reaction occurs 9.

As the pH decreases during esta process, both the rate of dentin demineralisation and the amount of dentin dissolved decrease. The irrigating solutions used in this experiment were of low concentrations. Comparison between two different pH values of citric acid and the chelating agents was essential to confirm its endodnocia at lower pH and concentration values. Few studies have evaluated its action using these parameters so far. However, EGTA did not cause erosion of the intertubular and peritubular dentin.

There was a molar concentration difference between all test solutions, but this difference was smaller among chelating agents. Note that the citric acid solution had almost endooncia the molar concentration when compared with the other solutions.

The concentration and association with other substances can be an important factor on the chelating agents’ action process. All acid solutions tested in this experiment are considered complexants and chelating agents. Combinations of decalcifying agents and -NaOCl solution have been recommended, because no single irrigator is capable of dissolving organic pulpal material and predentin as well as demineralising the inorganic calcified portion of the root canal wall.


The authors suggested that the acidity yielded by utilisation of chemical solutions on endodontic therapy could endoeoncia minimised by final flushing with water and use of calcium hydroxide sealers, which could neutralize this residual effect.

Besides, the application time should also be well controlled in the etching procedure. Attention to these factors will lower the risk of damage to the dental tissue during root canal cleaning and thus increase the success of endodontic therapy. Citric acid at neutral pH did not significantly change the calcium content of root dentin. Smear layer removal by EGTA. Efficacy of various concentrations of citric acid at different pH values for smear layer removal.

Effects of different pH values of citric acid solutions on the calcium and phosphorus contents of human root dentin. The pulpal response to dilute citric acid smear removers. J Oral Rehabil ; In vitro inhibitory effect of EGTA on macrophage adhesion: Braz Dent J ; The effects of citric acid concentration and application time. J Clin Periodontol ; Optimal citric acid concentration for dentinal demineralization. Endoroncia removal of the smear layer endoxoncia EGTA: J Clin Pediatr Dent ; Root canal irrigation with citric acid solution.

Received for publication on Sep 17, Sent for alterations on Oct 25, Accepted for publication on May 27, All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Services on Demand Journal. Procedure Forty-eight single-rooted freshly extracted human teeth with straight root canals and similar length were used in this study.

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